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All Volume Injection Method Procedure
The all volume injection method, the injection volume, the
feed volume, and the air volume are restricted by the following
relational expression. A stroke of the syringe capacity can't
be used all.
<175 µL of Standard syringe >
vi + vf + va ≦ 165 µL
vi :Injection volume
(The sample net volume to the flow path)
vf :Feed volume
(The wash fluid volume to deliver a sample to the loop)
va :Air volume
(The air volume of the lead and rear part of a sample)
100 µL loop is used for the autosampler as a standard. In
general, the feed volume is set as from 30 μl to 40 µL when
using 10 μl of sample.
In All volume injection method, the condition as [va: 0 μl of Air
volume] is recommended.
For a high accuracy gradient analysis, wash flow paths of the
buffer tube and the needle with the wash fluid2 after an
injection to avoid inflow of the wash fluid1 into the column.
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