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(6)
After confirmation of the above steps, connect the mobile
phase to the pump, and the detergent to the autosampler.
Be sure to use the mobile phase and detergent of the same
production lot.
(7)
Start the pump to fill each flow path in the pump and
autosampler with liquid.
(8)
After the mobile phase has reached the injection valve of
autosampler, turn on the autosampler power supply. If the
injection valve is switched over when no liquid is in it, the
valve seal may be damaged. To prevent this, be sure to
start the pump earlier.
The autosampler will go through the initialization sequence,
and then the initial screen will appear.
(9)
Press the FUNC key of the autosampler, and select
WPUMP to fill the flow path to the syringe valve with wash
fluid. (When two wash fluids are used, replace the wash
fluid2 side with fresh one). Then, make sure that there are
no air bubbles in the pipe from the wash pump to the
syringe valve.
(10) Press the FUNC key of the autosampler, and select
SPURGE to fill the syringe with wash fluid.
Then, make sure that there are no air bubbles in the
syringe and port4 of the syringe valve discharges waste
solution.
(11) Press the WASH key of the autosampler, and select
NEEDLE side to fill the needle and the injection port with
wash fluid1. (When two wash fluids are used, replace the
wash fluid2 side with fresh one).
Then, make sure that the detergent flows normally, there
are no leaks and the waste solution runs into the waste
bottle.
(12) Press the WASH key of the autosampler, and select RINSE
PORT side to fill the wash port with wash fluid1.
Then, make sure that the detergent flows normally, there
are no leaks and the waste solution runs into the waste
bottle.
4 - 4
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Troubleshooting
