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Amersham Typhoon scanner 5
Amersham Typhoon scanner NIR
For an overview of the types of lasers that are installed, refer to
Amersham Typhoon scanner, on page 132
Scanning modes
The Amersham Typhoon scanner can be used for scanning with three different scanning
modes.
Mode
Fluorescence
Phosphor imaging
Densitometry
Photomultiplier tubes
The photomultiplier tube (PMT) detects light and converts it to an electric signal. The PMT
potential (in volt) determines the amplitude of the signal.
Different types of photomultiplier tubes are installed in the Amersham Typhoon scanner:
•
•
Amersham Typhoon Operating Instructions 29193226 AC
Bi-alkali
Multi-alkali
Intended use
Fluorescence with five lasers, phosphor
imaging, and densitometry
Fluorescence with two lasers, 685 nm
and 785 nm, for NIR fluorescence appli-
cations.
and
Laser specifications, on page
Used for
Scanning fluorescent samples using different combina-
•
tions of lasers and filters.
Scanning luminescent samples, with all the lasers
•
turned off and no filters in use.
Scanning storage phosphor screens that have been ex-
posed to radioactive samples, to image and quantify radi-
oligands in the samples.
Scanning gels to obtain digital images of gels for doc-
•
umentation.
Densitometric imaging of stained gels (e.g., Coom-
•
massie-stained gels) for quantitation of samples.
3 System description
3.1 System overview
Main components in
132.
41
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