Outline; Isocratic System - Hitachi PRIMAIDE 1440 Instruction Manual

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1.1 Isocratic System

Primaide
Organizer
Primaide 1110

1. OUTLINE

The liquid chromatograph system consists of a pump for feeding
a mobile phase, a sample introducing section (autosampler,
manual injector) for injecting a sample, a column for
chromatographic separation of the injected sample, a column
oven for maintaining the column at a constant temperature, and
a detector section for detecting a signal of a separated
component of the sample.
In addition, a degassing unit for deaerating an eluent, a gradient
device for varying a mixing ratio of eluents with time, and other
optional devices are incorporated in the system.
The isocratic system has a simple configuration in which single-
solvent analysis is performed.
Wash Solution
Pump
Degassing
Drain
device
Fig. 1-1 Isocratic System
(1)
An eluent is fed by the pump through the degassing device.
(2)
A sample is injected from the autosampler.
(3)
The injected sample is separated through the column which
is maintained at a constant temperature by the column oven.
(4)
Each component separated from the sample is then
detected as a signal by the detector.
Mobile Phase
Column
1 - 1
Primaide 1440
Fluorescence
Detector
Detector cell
Primaide 1310
Column Oven
Primaide 1210
Autosampler
Drain

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