Prepare inlets and outlets
Follow the instruction below to prepare the inlets and outlets that are to be used in the
method run.
Step
1
2
3
Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB
Action
Connect inlet and outlet tubing to the ports that are to be used.
Immerse all inlet tubing that is to be used during the method run in the
correct buffers.
Immerse the waste tubing from the Injection valve, the pH valve, the Outlet
valve, and the Fraction collector into a sufficiently large waste vessel (typically
2-10 liters).
7 Prepare the system for a run
7.2 Prepare the flow path
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