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The stain-to-buffer ratio should be approximately 1:2 to 1:3, (i.e., the volume of buffer
should be about 2 to 3 times that of the stain).
Example:
1.6 mL of stain per 10 slides = 0.16 mL/slide
4.1 mL of buffer per 10 slides = 0.41 mL/slide
0.41 ÷ 0.160 = a ratio of 1:2.56 between stain and buffer
The rinse volume should be approximately 10 mL for the 10 slides.
A properly stained slide is the result of an interactive process involving the pump
volumes, the mixing process, and the stain-to-buffer ratio. A minimum ratio of 1:2 is
recommended; however, if the platen is filling properly, good mixing is occurring, and
the stained slides are acceptable under the microscope, the stain-to-buffer ratio should
not be adjusted.
5. When the determinations are complete, reconnect the three tubes to their appropriate
spouts and replace the waste tank.
Sensing Switch Fingers Adjustment
The sensing switch fingers must be properly positioned in order to accurately activate the
reagent pumps. If the reagents are not being dispensed, check the location of the fingers.
Not following recommended cleaning practices may force the sensing switch fingers out
of adjustment. Use the following procedure to adjust the sensing switch fingers.
1. Turn the instrument off and remove the line cord from the AC electrical outlet.
2. Raise the clear plastic lid on the instrument.
3. Loosen the two thumbscrews that are inserted through the notches in the back of the
circuit board cover.
Figure 6-17. Location of Thumbscrews
Page 6—18, Section 6: Minor Replacements and Adjustments
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